λ phage has a 20-hedral head and tail structure. The genome (48.5 kb) is a linear double-stranded DNA with 46 genes. The main structure of the outer shell is composed of the main coat protein gpE (415 copies) and is stabilized by the capsid protein gpD (402-420 copies). The head is connected to the tail of a flexible spiral formed by a disc of the main tail protein gpV. Both the tail protein gpV and the head protein gpD have been used for phage display. Initially, the lambda foo vector was constructed for C-terminal display on gpV, and its low display level makes it suitable for capturing high-affinity interactions. Later, a system for displaying peptides at the N-terminus or C-terminus of the major coat protein gpD was developed. Lambda phage has been designed to effectively display multiple copies of peptides, even large protein domains, providing powerful tools for screening peptide, protein and cDNA libraries1.
Fig 1. The genetic map of the λ phage. (Griffiths AJF, et al. 2000)
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The copy number of the foreign protein displayed by this system is an average of one molecule per phage, which indicates that the foreign protein or polypeptide may interfere with the tail assembly of the phage.
Protein D is more prominent in the head space structure, and is easy to bind with ligands, and can fuse foreign proteins with protein D. The feature of the protein D display system is that it does not need to secrete exogenous peptide or proteins into the bacterial cell membrane, and can display proteins that are toxic to cells.
Creative Biolabs can meet the needs of customers for providing λ phage display system construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
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