Monoclonal antibodies (mAbs) have become one of the most important biopharmaceutical product categories and continue to dominate the biopharmaceutical market in terms of approvals and sales. They are the most profitable single product category, and they represent six of the top ten best-selling drugs. Monoclonal antibodies (mAbs) are multifunctional biological macromolecules that can bind a variety of protein and non-protein targets with high specificity. These mAbs can be designed and produced in different forms to enhance their functions and uses. Antibody phage display can not only produce fully human antibody sequences, but it can also bypass some of the limitations of the immune system through in vitro affinity selection in a fully controllable biochemical environment. For example, phage display can provide antibodies that are not available in classical immunization, such as antibodies against very small or non-immunogenic molecules. The special advantage of controlling biochemical parameters at the moment of selection can also be used to shape the specific characteristics of antibodies from the beginning. By adding a soluble competitor during the affinity selection process of the immobilized antigen, unwanted cross-reactions can be eliminated.
Fig 1. Antibody phage display (APD) （Hammers C M, et al. 2014)
Creative Biolabs can provide phage display derived monoclonal antibody development solutions for researchers. We provide different types of analyses to evaluate antibodies after synthesis according to strict requirements. Our services are listed below, but are not limited to:
One of the most successful applications of phage display is the isolation of monoclonal antibodies from large phage antibody libraries. At Creative Biolabs, we provide three types of phage libraries, immune, naive, and synthetic antibody libraries.
In the second type of "single pot" library, antibodies are artificially constructed by in vitro assembly of V gene fragments and D/J fragments.
Although antibodies selected from many immunizations and even large single-pot libraries may be very useful to scientists, their affinity is often insufficient for therapeutic applications, such as immunotherapy, virus neutralization, or sensitive diagnosis. In Creative Biolabs, our scientists obtain sufficient apparent affinity antibodies by simply constructing multivalent molecules.
Phage antibody selection involves the continuous enrichment of specific binding phages from a large excess of non-binding clones. At Creative Biolabs, it is achieved through multiple rounds of phage binding to the target, washing to remove non-specific phage, and elution to recover specific binding phage.
According to the source of the library sequence, there are four types of phage display antibody libraries.
Creative Biolabs can meet the needs of customers by providing solutions for antibody development on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
