One of the major advantages of using in vitro or yeast-based genome modification is that phage genomes can be engineered without causing toxicity to the host. To generate functional phage particles from modified phage genomes in vitro or in yeast, researchers typically rely on transformation as a means of reintroducing the phage genomic DNA into the host bacterium, where the phage particles are then "primed". However, this process requires high transformation efficiency, especially for large phage genomes. While efficient transformation protocols have been devised for some bacteria, many other bacterial species are extremely difficult to transform. This constitutes a bottleneck for the throughput and efficiency of in vitro and yeast-based systems for phage genetic engineering.
Cell-free transcription-translation systems offer a potential solution to this problem. Cell-free transcription-translation systems can generate phage or virus-like particles from DNA in vitro, thereby overcoming the need for a highly competent bacterial host. For example, such systems have been used to replicate, synthesize, and assemble the T7 phage genome. In future work, it will be important to apply the strategy on a wider range of phage genomes.
Fig 1. Cell-free bacteriophage genome synthesis (Yeom H, et al. 2020)Our experienced genome scientists and quality experts are on hand to solve all your phage engineering questions. When you work with Creative Biolabs, we're fully focused, sharing our experience and knowledge, and responding to your needs in a timely manner. Our services are listed as follows, but are not limited to:
Phages can be modified to recognize non-bacterial targets, including eukaryotic proteins and tissues, by altering tail fibers or capsid proteins to include eukaryotic targeting motifs using phage display.
Phages can be associated with drugs with low water solubility, allowing for enhanced delivery and lower doses.
Phages have a large cargo capacity compared to most mammalian viruses, and as noted above, many phages can be readily engineered to express eukaryotic cell targeting motifs, which are also mediated by receptors endocytosis-mediated entry.
The ability to link genotype to phenotype using phage display technology makes phage a very powerful vaccine tool.
Creative Biolabs can meet the needs of customers by providing cell-free assembly of bacteriophage genomes services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
