CRISPR-Cas9 technology is an efficient, inexpensive, fast-to-design, and easy-to-use genome-editing tool that has been rapidly applied in many fields from basic biology to translational medicine. CRISPR-Cas9 technology was developed from a type II CRISPR-Cas system in which bacteria degrade target nucleic acids. CRISPR is the hallmark of most phage-resistant bacterial and archaeal genomes. The CRISPR-Cas system consists of two main parts: the Cas protein, as the catalytic core of the system, responsible for cutting DNA; and the CRISPR locus, as the genetic memory, directing the catalytic activity against foreign DNA. CRISPR loci typically consist of several discrete direct repeats separated by short variable DNA sequences obtained from extrachromosomal elements. CRISPR-Cas systems are currently classified into three main types (I, II, and III), characterized by distinct sets of cas genes, and are further divided into several subtypes. The IE-type CRISPR-Cas system has been used to enhance the engineering of the T7 phage genome. This method avoids scooping out a very small percentage of recombinant phage from a large number of wild-type phages.
Fig 1. CRISPR-Cas-based phage engineering. (Chen Y, et al. 2019)CRISPR/Cas9 is an RNA-guided targeted genome editing tool that allows researchers to perform gene knockouts, knock-in SNPs, insertions, and deletions in cell lines and animals. Creative Biolabs offers the best CRISPR/Cas9-mediated genome editing services. Our talented scientists have extensive experience in phage gene editing. They will work closely with you to help with microbial genome editing.
The CRISPR-Cas-Mediated Bacteriophage Genome Engineering technology has quickly become one of the simplest and most efficient genomes editing systems, offering several advantages:
Creative Biolabs can meet the needs of customers by providing CRISPR/Cas9-mediated genome editing services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
