DARPins (designed ankyrin repeat proteins) are genetically engineered antibody-mimicking proteins that typically exhibit highly specific and high-affinity binding to target proteins. They are derived from native ankyrin repeat proteins, the most common class of binding proteins in nature, and are responsible for a variety of functions such as cell signaling, regulation, and cellular structural integrity. DARPins consist of at least three repeating motifs or modules, of which the most N-terminal and the most C-terminal modules are called "caps" because they shield the protein's hydrophobic core. DARPins libraries are designed by sequence alignment of thousands of native ankyrin repeat motifs (~33 amino acids each) combined with structure-based design and recombinant DNA methods. These proteins are composed of repeating building blocks that form a stable protein domain with a large potential target interaction surface. DARPins correspond to the average size of native ankyrin repeat protein domains. Proteins with fewer than three repeats (i.e., capping repeats and one internal repeat) do not form sufficiently stable tertiary structures.

Our Services

Creative Biolabs provides custom scaffold library construction services for DARPins. Our innovative technology enables the generation of scaffold libraries with the desired diversity and high degree of controllability. Our services are listed as follows, but are not limited to:

• DARPins phage display library construction
• DARPins phage display library optimization
• Preparation for library panning and screening
• Bacterial plating, storing, and culturing
• Titering the phage display library
• Preparation of enriched library for repeat panning ELISA

DARPins Library VS. Antibodies Library

The advantages of DARPins over antibodies are as follows:

• Small size, stable over a wide temperature and pH range, low tendency to aggregate, and easy to produce in heterologous expression systems.
• Differences in DARPin and antibody paratope structures broaden the range of target molecules, and the ease of generating hybrid fusion proteins enables bispecific and multivalent constructs.

Our Capabilities

• All compounds are readily available. 10 mM DMSO solution pre-plate, easy to use.
• Validated by NMR and HPLC to ensure high purity and quality.
• Maximum structural diversity.
• Each compound represents a unique scaffold.

Our Advantages

• Our DARPins library is small size
• DARPins library has good solubility
• DARPins library is higher thermodynamic stability than commonly used antibody library
• Better tissue penetration
• DARPins library is relatively low cost

Creative Biolabs can meet the needs of customers by providing DARPins phage display library construction services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Reference

• Shilova O N, Deyev S M. DARPins: Promising scaffolds for theranostics[J].Acta Naturae (англоязычная версия), 2019, 11(4 (43)).

Please kindly note that our services can only be used to support research purposes (Not for clinical use).