Direct Isolation of Phage

The direct isolation method of bacteriophages is to extract bacteriophages from microorganisms and particulate matter in solid or liquid environmental samples. Then, through plaque detection, the extracted sample is used to infect the host bacteria. This method provides a snapshot of all phages present in the environmental sample that may infect the host. The combination of direct isolation and plaque assay is a rapid method for detecting phages in solid and liquid environmental samples. In fact, using direct isolation, you may find many different phages with different plaque morphologies. However, because not every phage in the sample can successfully infect the host bacteria, you may find as few as one plaque on the plate or no plaque at all. As a leading expert in the development of phages, Creative Biolabs specializes in the isolation and purification of various phages, including reporter phages, labeled phages and therapeutic phages. We provide customized production and purification services in a timely manner based on customer needs.

Our Services

In perhaps the most direct isolation method, environmental samples are sterilized to remove contaminating cellular microorganisms, and then directly inoculated onto one or more host strains and assessed for the appearance of plaques. At Creative Biolabs, our scientists can provide high-quality services to meet your needs. Our services are listed as follows, but are not limited to:

• Collecting environmental samples

By collecting samples from the soil, compost, water, or other bacteria-rich, our goal is to collect phages that can infect these bacteria. In order to increase the chances of collecting various phages that can infect specific host bacteria, we will consider the environment in which the host bacteria multiply.

• Direct isolation

This method extracts bacteriophages from microorganisms and particulate matter in solid or liquid environmental samples. And this method provides a snapshot of all phages present in the environmental sample that may infect the host.

• Plaque assay

The plaque assay allows you to visually confirm the presence of phage particles in the sample.

Direct Isolation Procedure

• Prepare workbench for aseptic work and assemble your supplies
• Collect environmental samples according to your needs.
• Extract bacteriophages from solid environmental samples (such as soil or compost). If your collection is a liquid sample, we will proceed directly to next step
• Prepare phage filtrate using the aseptic technique.

Direct Isolation Supplies

• Environmental samples
• Liquid culture medium (5 ml/sample)
• Sterile 3ml or 5ml syringe
• 0.22 μm syringe filter
• 5 ml serological pipette
• Microcentrifuge tube
• 15ml conical tube

Concentration Methods

Before plating, a variety of methods can be used to concentrate the sample, our concentration methods are included but are not limited to:

We Do Bettter

• We always give priority to quality assurance during direct Isolation of phage.
• We will provide detailed technical reports, including relevant experimental procedures and accurate and reliable experimental results.
• Creative Biolabs provides services covering all aspects of phage research.
• The team at Creative Biolabs is committed to meeting your needs as quickly as possible.
• We support global delivery.

Creative Biolabs can meet the needs of customers for providing direct isolation of phage services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).