The plaque count is considered the gold standard for phage enumeration. A single plaque is the result of a bacteriophage initially lysing a host bacterium, followed by subsequent bacteriophage killing neighboring cells. Conversely, the larger area of clearance may be the result of other antibacterial compounds or more phages mixed in the sample. The dual agar overlay assay (DLA) allows localized phage-host contact in a closed environment (petri dish) containing two layers of agar. The bottom layer is prepared with a bacterial growth-supporting medium containing 1-1.5% agar. The top layer contains the same medium, but with a lower concentration of agar, which is usually referred to as soft-agar and it is mixed with host bacteria and poured on the bottom layer to form a so-called lawn. If the phage can infect the tested bacteria, there will be obvious spots or plaques on the lawn, which contain lysed bacteria and phage particles. If the phage does not form clearly visible plaques, the agar may be replaced by agarose. The concentration (titer) of infectious phage in the phage stock solution or sample can be determined by spotting serially diluted samples on the bacterial lawn.
Infectious phages detection service is tailored to meet customers' specific needs. Creative Biolabs is a reliable partner dedicated to accelerating the speed to achieve customers' research goals. Our services are listed as follows, but are not limited to:
Enumeration of phages by the DLA method is a reference for the development of new methods. It allows the calculation of the number of phages that can complete the entire lysis cycle under test conditions, from binding to synthesizing and releasing new virus particles that infect neighboring host cells. It is simple and efficient, it can be implemented in any laboratory with minimal investment cost, and it is also suitable for many bacteriophages.
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Creative Biolabs can meet the needs of customers for providing phage enumeration and detection services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
