Compared with traditional optical microscopes, transmission electron microscopes (TEM) use electron beams to produce 1000 times the resolution. The increased resolution (down to 0.2 nm) is sufficient to visualize the virus. This technique can be used to quantify phage particles, although the sample needs to be highly concentrated (~106 particles/mL) to produce reliable results. Phage quantification using TEM is very accurate in determining the morphology and total number, but it is considered time-consuming, expensive, and impractical, unable to run many samples, and cannot be used for complex samples. In addition, sample preparation is cumbersome, and the technique requires skilled operators on complex instruments. Another technique for calculating the entire particle is flow cytometry. Here, the virus particles are labeled with fluorescent dyes and guided through the capillary. The small diameter of the capillary forces the particles to flow in a line, allowing detection of the light scattering caused by each particle. This method is fast and rigorous, so it is widely used. At Creative Biolabs, we provide TEM, flow cytometry, and double agar overlay assay (DLA), for customers to achieve customers' research goals.
Phage detection service is tailored to meet customers' specific needs. Creative Biolabs is a reliable partner dedicated to accelerating the speed to achieve customers' research goals. Our services are listed as follows, but are not limited to:
| Method | Basis of detection/enumeration | Duration | Manual labor | Advantages | Limitations |
| DLA | Virulent phage particles | 1-2 days | High | Simple, effective, | Slow, laborious, |
| TEM | Magnification of phage particles | 2-3 days | High | Works well with unknown phages | Costly, laborious |
| Flow cytometry | Phage particles | 4-12 h | Moderate | Apply in detection of different phages in a sample | Expensive, low sensitivity |
Creative Biolabs can meet the needs of customers for providing phage enumeration and detection services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
