Phage display technology is to clone the polypeptide or protein-coding gene or target gene fragment into the appropriate position of the phage coat protein structural gene so that the exogenous polypeptide or protein and coat protein are fused and expressed, and the fusion protein is displayed in the recombination of progeny phage. The phage vector pComb3 has been widely used in the construction of the natural phage antibody library and the expression of Fab antibody. The construction of the phage vector pComb3 is a fusion protein formed by linking the Fd chain of the antibody to the C-terminal region of the phage coat protein III (pIII). The Fd/pIII fusion protein and light chain protein genes are under the control of the sequence of the Lac promoter/controller, respectively, and are translated into proteins and then introduced into the bacterial cell membrane space. The heavy chain Fd is embedded in the membrane by fusion with pIII, while the light chain is secreted alone into the intermembrane space. The heavy and light chains assemble on the membrane to form active Fab antibodies.
We provide Fab phage display system construction services that will be customized for specific research questions. If your phage display system has been designed by yourself, just send us a request for a quotation of excel files containing phage display formation and we will check their feasibility. Please feel free to contact our experts to design the best Fab phage display system. Our services are listed as follows, but are not limited to:
Fab phage display technology has become a favorite tool for probing the spatial structure of proteins, exploring the interaction and binding sites between receptors and ligands, and finding ligand molecules with high affinity and biological activity. Research fields such as tumor therapy have had a profound impact.
Creative Biolabs can meet the needs of customers by providing Fab phage display system construction on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
