Phages are viruses that specialize in infecting bacteria. The interactions between phages and humans, animals, and bacteria play an important role in phage therapy research. The potential applications of phages in medicine, as well as general studies of the animal and human microbiome, have given rise to a need for developing tools that facilitate the study of phage circulation and deposition in tissues and cells. The use of fluorescently labeled phages can facilitate the detection of phages in mammalian cells and tissues. Molecular imaging can be very useful for studying phages in living systems. Fluorescent proteins are widely used biomarkers in cell biology research, especially in the visualization of protein localization and the monitoring of biological process dynamics. Providing phage with fluorescent proteins has the potential to be used for molecular imaging of phage. Labeled phage can be used to identify phage fate in vivo, e.g., phage translocation and retention in cells, tissues, and organs. The binding properties of phages are often used to create rapid bacterial detection assays. Phage detection of bacterial targets can also be accomplished using labeled phage components or labeled phage nucleic acid.
Fig 1. Scheme of red fluorescent protein (RFP) labelling of phages by phage display method. (Kaźmierczak Z, et al., 2021)The primary goal of phage research is to understand the interaction between phage and host cells in the context of infection. Advances in imaging techniques combined with genetic and genomic approaches have enabled the detailed characterization of phages and their interactions with host cells. Given the growing number of selective probes, labeling techniques, and available emission colors, fluorescence assays are particularly well suited for this multifaceted analysis. Our services are listed as follows, but are not limited to:
Phages radiolabeled with imaging radionuclides such as 99mTc may preferentially localize to sites of infection/inflammation.
Red fluorescent protein (RFP)-labeled Escherichia coli lytic phage as a new tool to study phage-cell and tissue interactions.
Luminescence-based enzyme-labeled phage assay is used for rapid detection of Escherichia coli serogroups.
Creative Biolabs can meet the needs of customers by providing fluorescent labeling of phage design and production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
