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Full Length cDNA Library Construction

Full Length cDNA Library Construction

The cDNA library is based on a specific tissue or mRNA as a template, and then the complementary cDNA formed by reverse transcription is ligated with an appropriate vector (commonly used phage or plasmid vector) and transformed to form a recombinant DNA clone group, which contains all the mRNA information of the cell. The cDNA library specifically reflects the protein coding gene expressed in a certain tissue or cell at a specific developmental stage, so the cDNA library has tissue or cell specificity. cDNA library has special advantages in studying the expression status of the genome and the function identification of expressed genes in specific cell, so that it can be used in the study of ontogeny, cell differentiation, cell cycle regulation, cell aging and death regulation. The cDNA library has a wider application and is the most commonly used gene library in research work. Creative Biolabs provides faster, cheaper and better cDNA library construction services. Our professional design team will ensure you get what you need.

Our Services

Creative Biolabs has a group of outstanding library construction professionals who can provide standardized cDNA library construction, EST sequencing and analysis services, and library construction for tissues and cells such as animals, plants, microorganisms, etc.

  • Full length cDNA library construction
  • QC of cDNA library
  • Check the library capacity

Features

  • 1x107 primary clone
  • Receive two size fractionation libraries (0.4 kb-2.0 kb and 2.0 kb and above)
  • 90-100% property
  • Q.C. by sequencing
  • Phage resistant host competent cells for reproduction
  • 250-500 micrograms of endotoxin-free DNA library are provided
  • Used for deliberate semi-reflective procedures to prevent biased expressions
  • Fast turnaround time

Workflow

  • RNA extraction
  • mRNA isolation
  • cDNA synthesis and homogenization
  • cDNA length classification
  • All-direct recombination of graded cDNA and target vector
  • Electrotransformation of recombinant products

Application

Full Length cDNA Library Construction
  • Functional gene filter
  • Discovery of new genes
  • Analysis of biological genetic function
  • Biological resources development and protection
  • Biological research, cell development, aging and death and other life phenomena

Creative Biolabs can meet the needs of customers for providing full length cDNA library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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