Homologous recombination (HR) is a type of genetic recombination that occurs during meiosis. HR is the genetic result of the physical exchange between two separate chromosomes or two aligned regions of the same DNA on the same chromosome. HR mainly occurs between homologous chromosomes with different markers around the exchange region. Exchanges sometimes occur between non-homologous chromosomes, or within the same chromosome, between the same regions, called "duplications," allowing physical detection of recombination. One of the most common and well-established methods for engineering phage genomes is through homologous recombination in their bacterial hosts, which can occur between two homologous DNA sequences as short as 23 bp. Mild phages can be stably maintained in bacteria so that their genomes can be modified using the same methods as bacterial genetic engineering, but the genomes of virulent phages cannot be fully cloned into bacteria for subsequent genetic manipulation due to cytotoxicity, etc. Therefore, virulent phage genomes are usually edited by homologous recombination (allelic exchange), first, DNA edited is integrated into a plasmid and transformed into the host bacterium, and then the strain is infected with wild-type phage so that the phage gene is combined with the plasmid homologous recombination is performed on the designed DNA fragments.
Fig 1. Phage engineering via homologous recombination. (Pires D P, et al. 2016)We have been supporting global clients in the Americas, Europe, Asia, and Australia, from academic laboratories, small biotech start-ups to global biopharmaceutical companies. Whether you wish to entrust complete phage production to a partner or manage capacity bottlenecks with a single project, you can benefit from our professional services. Our services are listed as follows, but are not limited to:
Remove some non-essential genes and add genetic elements, or modularly design the entire phage genome, and split the designed genome sequence into Oligo sequences of about 100 nt that are conducive to synthesis
Assemble complete phage genomes by yeast recombination platform, PCR or Gibson
Use electroporation, cell-free expression system or l-type cells to rescue the synthetic phage genome into a complete phage genome.
Preliminary characterization of synthetic phages by one-step growth and inhibition curves.
Creative Biolabs can meet the needs of customers by providing homologous recombination-mediated phage genome engineering services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
