Hybrid phage vaccines are produced by a combination of phage display and phage DNA vaccines. In the hybrid phage vaccine model, both peptide vaccines and DNA vaccines are delivered in a phage structure.The latest development of phage-based vaccines has focused on improving the interaction and internalization of phage particles through antigen-presenting cells (APC) to increase the immune response. The system relies on phages displaying proteins or peptides with high affinity to antigen-presenting cells or the antigen itself, and they carry eukaryotic expression cassettes encoding specific antigens on their genomes. The ultimate goal is to enhance the immune response by combining two effects. For these reasons, this strategy has been proposed as a targeted therapy based on its ability to deliver antigen genes to immune response activators.
Creative Biolabs can provide high-quality hybrid phage vaccine development services. We provide different quantitative analyses and qualitative analyses to evaluate hybrid phage vaccine according to strict requirements. Our services are listed below, but not limited to:
Our professional scientists have extensive experience in phage display technology. The types of display systems we used in the vaccine development process are listed in the Table 1.
Table 1 Features of phage display systems (Gao J, et al. 2010)
| Phage | Genome (size) | Display sites | Advantages |
|---|---|---|---|
| Filamentous phage | Single-stranded looped DNA (6.4 kb) | N-terminal of p3 C-terminal of p3-JUN/Fos C-terminal of p6 N-terminal of p8 |
P3 can be used to screen high affinity ligands by univalent display. P8 has the capacity to display proteins in high copy number |
| Lambda phage | Double-stranded linear DNA (50 kb) | N- or C-terminal of pD, C terminal of pV | Capable of displaying complicated, high molecular weight proteins |
| T4 phage | Double-stranded linear DNA (160 kb) | C-terminal of SOCcodonN-terminal of HOC | Capable of displaying larger proteins in high copynumber; displaying inserts with stop |
| T7 phage | Double-stranded linear DNA (39 kb) | C-terminal of cp10 | Displaying small peptides in high copy number and larger peptides or proteins in low or mid-copy number; displaying inserts with stop codon |
Creative Biolabs can meet the needs of customers by providing vaccine development services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
