Hyperphages carry the pIII gene deletion. They are produced by an E. coli packaging cell line that produces a functional pIII for packaging phage genomes with pIII deletions. The resulting hyperphages carry functional pIII on their surface but lack the pIII gene in their genome. These hyperphages can then be used to infect bacteria with phagemid libraries. Each resulting display phage carries multiple copies of the antibody or peptide on its surface, significantly increasing the panning efficiency. A helper phage technology ("Hyperphage System") has been developed by Rondot et al. Hyperphage system allows for improved antibody presentation in phage display by increasing the number of antibodies displayed per phage particle (up to 5 vs. 0.01), so this system offers great advantages in functional gene analysis and proteomics. Phage panning can be performed with small amounts of antigen and with greater efficiency. For example, the use of universal libraries for antibody isolation can be improved by panning hyperphage libraries on Western blots following two-dimensional gel electrophoresis.
Fig 1. Schematic representation of gIIIP protein encoded by the helper phages AGM13 and VCSM13. (Gupta A, et al., 2013)Based on years of expertise, we can use our in-house phage and antibody libraries to advance your project. Our collaborative approach and broad scientific expertise in hyperphage display can advance your project from a hyperphage library to downstream production, characterization, and development of appropriate biologics based on your needs. Our services are listed below, but are not limited to:
Large numbers of open reading frames (ORFs) can be analyzed by panning against synthetic membrane-bound peptide epitopes. In cancer research, hyperphage libraries can be a tool to discover new tumor markers by translating them to the cell surface.
Creative Biolabs can meet the needs of customers by providing hyperphage display system construction on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
