Hyperphage is an alternative to the M13KO7 helper phage for antibody phage display. It can improve the efficiency of antibody display on phage by 2-3 orders of magnitude. It is achieved by forcing packaging E. coli cells to exclusively use the pIII portion of the antibody fusion protein for virion assembly. The latest breakthrough in enhancing the performance of antibody phage display is the development of hyperphage technology. By using this method, antigen-binding activity is increased approximately 400-fold by forcibly displaying oligovalent antibodies on each phage particle. Packaging of the universal human scFv library using hyperphages improved the specificity enrichment factor: after two rounds of panning, more than 50% of the isolated antibody clones bound to the antigen, compared to 3% using the conventional M13KO7 helper phage. Thus, hyperphages are particularly useful in stoichiometric situations, where the chance that a single phage is known to be able to localize the desired antigen is low. In particular, novel tumor markers can be detected by allowing panning on the cell surface with greater sensitivity. In the search for novel targets for gene or drug delivery in a tissue- or cell-specific manner, in vivo panning is expected to benefit from hyperphage packaging.
Hyperphage is compatible with all common phagemid systems that use full-length pIII gene fusions into conjugate libraries and has been extensively validated to improve the panning of antibody and scaffold libraries. Our team has been optimizing hyperphage production process and making the final product fully compliant with regulatory and technical process requirements. Our services are listed as follows, but are not limited to:
Hyperphage system allows for improved antibody presentation in phage display by increasing the number of antibodies displayed per phage particle (up to 5 vs. 0.01), offering great advantages in functional gene analysis and proteomics. Phage panning can be performed with a small amount of antigen and is more efficient. For example, the use of universal libraries for antibody isolation can be improved by panning blots of protein spots using hyperphage-packaged libraries after 2-dimensional gel electrophoresis.
Creative Biolabs can meet the needs of customers by providing hyperphage production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
