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Phage M13 Production

M13 is one of the Ff phages (fd and f1 are other phages) and is a filamentous phage family member. Phage M13 is a non-lytic virus approximately 6.6 nm in diameter, 880 nm in length, and covered along its length with 2,700 major coat proteins (pVIII). The M13 plasmid is used in many recombinant DNA processes, and the virus is also used in phage display, directed evolution, nanostructure and nanotechnology applications. To meet these needs, our scientists examined vital process parameters during phage propagation to increase phage productivity. Temperature, harvest time, media composition, feeding regime, phage and bacterial concentrations were analyzed in a scaled-down system. It was found that temperature, medium composition and feeding regimen had greater effects on phage production than other factors. The temperature has the opposite impact on bacterial growth and phage production. As the undisputed pioneer and innovation explorer in manufacturing, Creative Biolabs has established a full range of professional services based on our phage generation services. Among them, Creative Biolabs is proud of our excellent phage M13 large-scale production service, which can generate high-quality phage M13 for global customers.

Surface charge properties of the bacteriophage M13Fig 1. Surface charge properties of the bacteriophage M13 (Passaretti P, et al., 2020)

Our Services

Creative Biolabs is the world's leading phage production manufacturer due to its many years of experience in phage manufacturing, other biologics production, and large-scale production. Creative Biolabs' custom development and manufacturing services have helped developers worldwide to accelerate research and open their laboratories to breakthrough science and breakthrough discoveries. Our services are listed as follows, but are not limited to:

  • Phage M13 production
  • Development of phage M13 manufacturing processes
  • Stability studies of phage M13
  • Assessment of phage M13 lysis efficiency
  • Effects of temperature, pH and ultraviolet radiation
  • Adsorption rates assay phage M13
  • Phage M13 purification
  • Phage M13 modification

Our Capabilities

  • Possible deliverables: crude phage lysate, concentrated crude phage lysate, purified concentrated phage material, various intermediates
  • Upstream scale: erlenmeyer flask, 1 L, 15 L, 50 L, continuous
  • Downstream scale: downstream capacity matches the fermentation scale. This means that one fermentation batch can be purified in one run, without the need for batching
  • Downstream options: gel filtration, diafiltration, affinity,etc.
Advantages

Our Advantages

  • Phage related project tailored to suit your needs
  • Independent quality department to ensure each phage compliance
  • Transparent processes and weekly project progress reports of your phage M13 production
  • Professional project reports ready for regulatory filing
  • Fast turnaround time and competitive pricing of phage M13 production

Creative Biolabs can meet the needs of customers by providing phage M13 production services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Reference

  • Passaretti P, Sun Y, Dafforn T R, et al. Determination and characterisation of the surface charge properties of the bacteriophage M13 to assist bio-nanoengineering[J]. RSC Advances, 2020, 10(42): 25385-25392.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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