Helper phages (e.g.M13K07) provide all the necessary gene products for particle formation, when using phagemid vectors. They are mutated wild-type bacteriophages that contain the entire genome, with defective replication origins or packaging signals, and are therefore inefficient at self-packaging. When infecting E. coli containing phagemids, phage progeny are produced and only the phagemids are efficiently packaged. M13KO7 is a derivative of M13 with an origin of replication from P15A. M13KO7 is a helper phage designed to produce single-stranded plasmid DNA. M13KO7 is able to replicate in the absence of phagemid DNA. In the presence of phagemids with wild-type M13 or f1 origin, single-stranded phagemids are preferentially packaged and secreted into the medium. It allows for easy production of single-stranded phagemid DNA for mutagenesis or sequencing. The amplification of M13K07 helper phage requires the use of E. coli TG1, ER2738, or strain XL1-Blue MRF', as these strains contain the supE44 mutation that provides glutamine-inhibiting tRNA. The M13K07 helper phage is strictly used to generate single-strand DNA from excised phagemids.
The extremely high concentrations, unique quality control and reliability of developed by our team make them the source of choice for phage display applications.
The phage DNA concentration is determined by UV spectrophotometry and the virion concentration was calculated based on the length of the M13KO7 genome.
In order to extract the proliferating helper phage from the host microorganism during production, cell lysis must be performed, which results in the growth medium being saturated with nucleic acids and intracellular components, making the solution viscous and difficult to handle and purify. However, at Creative Biolabs has extensive experience to deal with these problems. Our advantages are listed as follows:
Creative Biolabs can meet the needs of customers by providing M13KO7 helper phage production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
