Max Helper Phage Production

Max helper phage was obtained by modifying M13KO7 to delete the pill gene ( M13 K07ΔpIII ), produced by a functional pill-producing E. coli packaging cell line. Max helper phage carries functional pIII on its surface but lacks the pIII gene in its genome. Due to the lack of pIII gene, when using max helper phage to build a phage library, the generated phage must use the pIII fusion protein on the phagemid vector, so the packaged phages display the target protein without an empty load. Several copies of a protein or peptide can be displayed on the surface of each phage, max helper phage improves protein presentation in phage display by increasing the number of proteins or peptides displayed per phage particle (up to 5 to 0.01 compared to normal Helper Phage) Effect. Phage panning can be performed with a small amount of target protein and is more efficient. Max helper phage and hyperphage have the same effect.

Our Services

Creative Biolabs is committed to providing max helper phage production services, and we have been designing, synthesizing, and applying helper phage technology for many years. You can use our free design assistance service to take advantage of this knowledge. We provide max helper phage services that will be customized for specific research questions.

• Design of continuous max helper phage production process
• Construction of max helper phage
• Max helper phage production
• Max helper phage isolation
• Max helper phage purification
• Max helper phage identification
• Max helper phage quality control
• Max helper phage stability tests
• Max helper phage sensitivity assay
• Max helper phage MOI determination
• Max helper phage Uv inducation determination
• One-step growth curve of max helper phage
• Determination of max helper phage trypsin sensitivity and immunoblotting
• Max helper phage quantification and reactivity determination by ELISA

Key Features

• Max helper phage is used for phage display
• Tool for isolation of recombinant antibodies, proteins and peptides
• Increased panning efficiency
• Panning with reduced amount of panning antigen
• Identification of high and low affinity binders
• Max helper phage can infect F+ bacteria
• The pIII gene is lost in the max helper phage genome
• Max helper phage carries the kanamycin resistance gene in the genome

What We Offer?

Creative Biolabs has many years of experience in synthesizing and using max helper phage. We provide our customers with:

• Customized synthesis of max helper phage library size, format and synthesis scale.
• Various max helper phage modification, including cysteine modification, N-terminal alanine modification, aspartic acid and glutamic acid modification, N-terminal serine and threonine modification and more.
• Free design assistance from the experts with rich experience and expertise.
• Fast and reliable custom synthesis, global delivery.

Our Advantages

Creative Biolabs can meet the needs of customers by providing max helper phage production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).