Determining the attachment rate in bacterial cells is the basis of phage ecology research and it is used to estimate the impact of predators (phages) on prey (host cell) populations. The first step in phage infection is to attach phage virus particles to susceptible host cells. This adsorption process is usually described by mass-action kinetics, which implicitly assumes that the bulk density and adsorption rate have equal effects on the adsorption process. Therefore, it can be considered that an environment with a high host density is equivalent to a phage with a high adsorption rate, and vice versa. In the one-step growth experiment and transcription study of phage-infected cells, it is also important to ensure the synchronization of phage infection. In 1931, Krueger1 demonstrated that the binding of phage particles to bacterial cells (live or dead) follows first-order kinetics that can be defined by the following equation:
It provides a practical method for studying the adsorption rate of bacteriophages to cells and the interaction between these viruses and their surface receptors.
Creative Biolabs is a biotechnology company specializing in providing measurement of phage adsorption rate services. Our biological analysis service department has a professional scientific research team, the analysis laboratory is equipped with advanced instruments and equipment, and comprehensive information management is implemented. Our services are listed as follows, but are not limited to:
The goal of phage strain construction is to construct isogenic phage strains that differ in traits, such as adsorption rate, lysis time, and marker state.
Our award-winning DNA sequencing services provide high-quality results
The adsorption rates are estimated by fitting the data with the model of equation.
A standardized method developed by our experts in phage used to determine the lysis time for each phage.
We provide phage data analysis assistance for academic and commercial customers who focus on quantitative research.
Phage adsorption generally includes three steps: initial contact, reversible binding, and irreversible attachment. The first step involves random collisions between the phage and the host caused by Brownian motion, dispersion, diffusion, or flow.
The test sample can be from fermentation broth, air, sewage, soil, etc.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
