One-step Growth Curve of Phage

The one-step growth curve is an experiment to study virus replication. It was originally established to study phage replication and has now been extended to the study of animal virus and plant virus replication. Taking advantage of the phage infection cycle, the growth curve of phages can be obtained under laboratory conditions. The operation is to inoculate an appropriate amount of phage in high-concentration sensitive cell culture, or a high-fold dilution of the phage-cell culture, or treat the phage-cell culture with an antiviral serum to establish a simultaneous infection. The infection time is the abscissa while the phage titer is the ordinate and it is used to determine the time interval between phage infection and mature virion release and to estimate the growth curve of the new phage particles released by each infected cell, which is a one-step growth curve. The one-step growth curve is divided into latent period, lysis period, and stationary period. This curve is of great significance for understanding and studying the characteristics of phages.

Our Services

One-step growth curves are used to make determinations about the life cycle of a virus on a particular host. Besides, One-step growth curve experiments are also used to determine the latent time and phage burst size. By following a virus infection during one life cycle phase of host a growth curve can be constructed and the burst size can be calculated. Our services are listed as follows:

• Host growth curve
• Phage lysate titer determination
• One-step growth experiment
• The size of the phage outbreak determination
• The latent period, lysis period and stationary period of phage determination

Other Related Services

• Bacterial strains isolation
• Isolation and purification of phages
• Determination of the lysis spectrum of the isolated phages
• Determination of multiplicity of infection
• Determination of the efficiency of plating (EOP values) for the selected phages
• Phage DNA extraction and restriction endonuclease analysis

Our Advantages

• Creative Biolabs has been forming the stereotype of biological science research by discovering, developing and supporting excellent phage research services.
• From the beginning of our company's cultivation, scientific progress and environmental management have been our highest priority.
• Our phage service is a high-quality service tailored to customers.
• Our phage service is very safe and the isolated phages will not affect beneficial bacteria, animal, or human cells. There is no food chain effect like antibiotics, and no super bacteria will be produced.

Creative Biolabs can meet the needs of customers for providing one-step growth curve services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).