Phage Display Combinatorial Peptide Library Construction

Selection from phage-displayed combinatorial peptide libraries is an efficient strategy for identifying peptide ligands of target proteins. Existing protocols for the construction of phage display libraries utilize ligation to double-stranded phage DNA. Peptides have several advantages over traditional compounds, but also some disadvantages. On the positive side, they are highly specific and efficient because they mimic the smallest possible part of a functional protein. They also have other very interesting therapeutic properties, such as high biodegradability of peptidases present in the body, limiting their accumulation in tissues, and leading to lower toxicity since their degradation products are amino acids. Potential disadvantages of using peptides are limited ability to cross membrane barriers, short half-life (rapid blood clearance) and potential immunogenicity, complex mode of action, high cost of production, and poor oral absorption, often requiring intravenous administration. However, it is possible to overcome these limitations by using our new technologies to modify these molecules and their delivery, stability, and application in preclinical settings. For example, the d-peptide is resistant to natural proteases (unlike the l-peptide), has a longer half-life in serum, is efficiently orally absorbed, and is recommended for therapeutic use.

Fig 1. Application of biopanning with phage-displayed random peptide libraries. (Wu C H, et al., 2016)

Our Services

Phage display combinatorial peptide libraries are powerful research tools for high-throughput screening of protein interactions. Of all the molecular display technologies available, phage display has proven to be the most popular method. Our services are listed as follows, but are not limited to:

Combinatorial peptide library construction
Distribution analysis of phage display peptide libraries
Screening by phage display
Illumina sequencing
Peptide synthesis
In vitro antiviral assay

Our Methods of Cloning Libraries of Peptide Genes Fused to the Phage Coat Protein Gene

Method I: it is based on the fusion of a gene encoding a peptide or protein with a phage capsid protein in an expression vector called a phagemid.
Method II: it is to insert the chimeric gene directly into the phage genome. This is a simpler process as infection with helper phages is not required.
Method III: the phage genome carries two genes encoding two different types of proteins: one recombinant and the other wild-type. The resulting phage coat is a mosaic displaying both wild-type and recombinant proteins.

Phage Displayed Combinatorial Peptide Library Application

Phage-displayed combinatorial peptide libraries can be used for:

B cell and T cell epitope mapping
Selection of bioactive peptides that bind to receptors or proteins, disease-specific antigen mimics, peptides that bind non-protein targets, cells - specific peptides, or organ-specific peptides
Development of peptide-mediated drug delivery systems and other applications.

Creative Biolabs can meet the needs of customers by providing phage display combinatorial peptide libraries construction services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Reference

Wu C H, Liu I J, Lu R M, et al. Advancement and applications of peptide phage display technology in biomedical science[J]. Journal of biomedical science, 2016, 23(1): 1-14.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).