Phage Display Cyclic-Peptide Library Construction

Superior to linear peptides in biological activity, cyclic peptides are considered to have great potential as therapeutic agents. To identify cyclic peptide ligands for therapeutic targets, phage-displayed peptide libraries cyclized by covalent conjugation of cysteines have been widely used. Genetically encoded libraries of cyclic peptides are increasingly used to find macrocycles. Macrocyclic peptides are ideal for targeting protein-protein interactions. Such libraries can be generated by a variety of methods, such as phage display, mRNA display, and split intein loop ligation. Bicyclic peptide phage libraries can be further generated by chemical cross-linking. Phage display benefits from large libraries and ease of experimentation. By designing random peptide sequences flanked by cysteine residues, highly diverse cyclic M13 peptide-phage display libraries can be generated. However, the reversibility of disulfide bonds limits the applicability of these macrocycles in the intracellular environment. Phage display is a powerful technique often used to generate peptide- or protein-based ligands. The success of phage display selection critically depends on the size and structural diversity of the library, but the generation of large libraries remains challenging.

Fig 1. Macrocyclic peptides for the win. (Simonetti L, et al., 2020)

Our Services

Creative Biolabs is a leading CRO providing phage-displayed cyclic peptide library construction development, assay validation, and sample testing services to support global phage development and manufacturing activities. Our services are listed as follows, but are not limited to:

Macrocyclic peptide library design and construction based on phage display
Bicyclic peptide library design and construction based on phage display
Design of a cyclic peptide library with high backbone diversity on phage display
Calculating phage clones and library expansion
Phage panning experiments
Peptide cyclization
Phage production
Cyclization of cysteines on phage
Chemical synthesis or biosynthesis of peptides
Peptide purification by HPLC

Our Capabilities

The libraries range in size from 2.1 x 10⁸ to 1.8 x 10¹¹ clones, The recombination rate is 68-100%.
These libraries display cysteine-restricted or focused (fixed residue) peptides ranging in length from 8 to 20 amino acids.
The cloned library is based on 273 different peptide backbones and therefore has great backbone diversity.

Our Advantages

Creative Biolabs has several competitive advantages in both peptide production and phage display.
Excellent company references, related to work in peptide generation
Specific knowledge and techniques
Project integration, from production or supply, project management to implementation.
In the process industry, our strength is a good reference from the biological industry, offering comprehensive services that integrate design, production and implementation into one business model.

Creative Biolabs can meet the needs of customers by providing phage display cyclic peptide library construction services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Reference

Simonetti L, Ivarsson Y. Genetically encoded cyclic peptide phage display libraries[J]. 2020.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).