Phage Display Cys-constrained Random Peptide Library Construction

We developed a unique phage display technology to discover new highly restricted peptides. Our proprietary Cys-constrained random peptide library construction technology generates highly constrained redox-stable peptides with enhanced affinity, selectivity, and proteolytic stability for diagnostic and therapeutic applications. Phage display is a technique that enables de novo discovery of peptides against any target of interest by exploring DNA-encoded peptide libraries. Phage is a virus that infects bacterial cells. We can identify peptide binders for a given target by mimicking the evolutionary process. This powerful technology can screen a library of billions of different peptides in less than four weeks. However, the ability to screen such a large library does not guarantee the identification of lead peptides for the protein of interest. This is because the search must also include the correct chemical space, which is known to be highly target-dependent. Thus, we can probe different parts of conformational peptide space, since binding to different proteins requires multiple complementary shapes at the peptide level.

Our Services

Our phage display peptide library is a collection of high-complexity libraries (10⁹) in Cys-constrained format. They also include a library of conformational peptides to support any requirement. Our services are listed as follows, but are not limited to:

• Cys-constrained random peptide library construction in M13 pVIII- display formats
• Cys-constrained random peptide library construction in M13 pIII-display formats
• Cys-constrained conformational HC-CDR3-based peptide library construction in M13 pVIII-display formats
• Cys-constrained conformational HC-CDR3-based peptide library construction in M13 pIII-display formats
• Loop-constrained octapeptide peptide phage display library construction

Our Capabilities

• Our peptide discovery team has developed a synthetic technology platform that integrates automated peptide synthesizers, state-of-the-art microwave technology and ligation technology to provide peptide tools.
• We provide selection and screening design according to specific project requirements.
• We provide recombinant protein, on/intracellular, in vivo.
• We provide screening by monoclonal analysis and/or NGS.
• We provide binding and/or functional assays.
• We provide chemical SAR and modification for in vivo stability studies.
• Utilizing state-of-the-art technology, our experienced DMPK team conducts in vitro and in vivo studies.

Our Advantages

• Our peptide discovery experts apply Creative Biolabs' proprietary peptide technology, peptide chemistry expertise, and our proven track record of contract research and R&D collaborations to our comprehensive peptide library construction services.
• Our team can screen libraries of over a billion members in days, providing an efficient alternative to more traditional epitope mapping, receptor-ligand recognition or protein evolution approaches.
• Creative Biolabs can provide customers with phage display technology covering a wide range of technologies, including library construction and screening.
• Phage display technology to screen targeted peptides for the development of anticancer drugs can definitely provide new opportunities for the treatment of many tumor diseases. We are confident that Creative Biolabs' peptide library screening service can open up more possibilities for your research.

Creative Biolabs can meet the needs of customers by providing phage display Cys-constrained random peptide library construction services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).