Proteases are an important class of enzymes whose activity is critical for many physiological and pathological processes. These enzymes are involved in a variety of biological functions, including tissue remodeling and morphogenesis, infection, coagulation, neoplasia, and cancer metastasis. Protease activity can serve as a valuable diagnostic biomarker and therapeutic target. Given their importance and diversity, there is an unmet need for unbiased techniques for the independent analysis of protease activity and as components of complex biological mixtures. Only a small subset of the physiological substrates of proteases have been characterized in some detail. Currently, unbiased protease analysis methods are based on mass spectrometry and tend to be cumbersome and expensive. Targeted activity-based assay techniques can detect active proteases, but are typically limited to low levels of assay multiplexing and are limited to enzymes with well-characterized substrates. At Creative Biolabs, our team used phage display of random peptide libraries to characterize cleavage motifs to overcome these problems.
We developed a protease detection platform based on DNA analysis detection technology combined with phage display technology to detect endopeptidase activity. Our professional laboratory staff have advanced skills and extensive experience in handling projects in different fields, and can ensure that data from customers from different disciplines can be customized for protease analysis.
Creative Biolabs can meet the needs of customers by providing protease activity assay development solutions on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
