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Phage Display Naïve Library Construction

Phage Display Naive Library Screening

The phage display library solution we provide not only provides an antibody library with a capacity of up to 108, as also even provides antibody library screening services according to customer needs. The most common request is the VHH single domain antibody library screening service from immunized alpaca or llama. We can assist researchers in the procurement of antigens and immunization strategies and library construction and phage display library screening. The deliverable can be simple sequence information of the selected antibody or a small amount of soluble purified antibody. Our phage display vector and display system are unique and have many advantages. We have also made custom scFv libraries from mice and humans, and can make various other sources (rabbit, sheep, cow), as long as antibody sequence information is available.

Our Services

The phage display platform currently developed by Creative Biolabs scientists covers the immune antibody library, natural human antibody library, and other types of antibody libraries, using mature screening strategy. It can carry out in vivo screening and Ex vivo screening, plus accurate sequence analysis and evaluation methods, forming an efficient antibody panning technology platform, which greatly improves the efficiency of antibody screening. Our services are listed as follows, but are not limited to:

  • SDS-PAGE or other methods to detect antigen quality
  • Human or mouse origin, 108 to 109 storage capacity
  • 4 rounds of panning, antibody affinity exceed 10-7
  • Phage amplification
  • ELISA test
  • Phage DNA extraction
  • Phage DNA sequencing
  • Antibody detection
  • Experiment report analysis
  • cDNA of antibody gene fragment

Screening Strategies

  • Solid-phase antigen-antibody library screening strategy
  • Liquid phase antigen screening strategy
  • In vivo screening
  • Ex vivo screening

Workflow

Phage display naïve library screening workflow

Standard Delivery Content

  • Antibody sequence
  • Antibody library
  • Phage vector
  • Experimental report

Creative Biolabs can meet the needs of customers for providing phage display naïve library screening services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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