Phage Display Peptide Library Screening

Peptides are two-dimensional linear amino acid chains, usually very short in length (less than 50 AA). They are either designed through a reasonable calculation method, or through phage display screening to obtain peptides that highly specifically bind to the target, and may modulate the target. Compared with antibodies (~150 kDa), peptides are relatively small (~3–5 kDa), so they are easy to synthesize and modify, have higher cell membrane permeability, and have lower immunogenicity. In cancer treatment, these peptides can be used as targeting ligands to help specifically deliver cytotoxic drugs to the tumor vasculature, tumor microenvironment, or cancer cells. On the other hand, peptides can also be delivered intracellularly to target cancer to specifically upregulate transcription factors, oncogenes, or enzymes. Phage display is a powerful technique for screening and isolating target-specific peptides. This method uses phage to display foreign peptides or antibodies on its surface by inserting genes encoding corresponding polypeptides into the phage genome.

Our Services

As a recognized expert in phage display technology, Creative Biolabs provides high-quality services in the screening of phage display peptide libraries. In order to select peptide with high affinity and specificity, we have developed a series of selection strategies.

• Phage libraries and biopanning
• Amplification and sequencing of the selected phages
• Peptide synthesis and labeling
• Peptide-based immunofluorescence assay

The General Scheme of Phage Display Technique and Biopanning Selection of High Affinity Peptide

• The first step: library construction & amplification
• The second step: target capturing step
• The third step: remove unbound & nonspecific phages
• The fourth step: competitive elution
• The fifth step: infection stage

Fig 1. The general scheme of phage display technique and biopanning selection of high affinity peptide. (Saw P E, et al, 2019)

The Advantages of Peptide as Compared to Antibody

Application

• B cell and T cell epitope mapping
• Selection of biologically active peptides that bind to receptors or proteins, disease-specific antigen mimics, peptides that bind to non-protein targets, cell-specific peptides, or organ-specific peptides
• Development of peptide-mediated drug delivery systems and other applications

Creative Biolabs can meet the needs of customers for providing peptide library screening services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Reference

• Saw P E, Song E W. Phage display screening of therapeutic peptide for cancer targeting and therapy[J]. Protein & cell, 2019, 10(11): 787-807.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).