Unlike natural and immune libraries, semi-synthetic libraries consist of a mixture of natural and chemically synthesized sequences. Gene synthesis methods are commonly used to provide random complementarity determining region (CDR) combinations. The advantage of semi-synthetic antibody libraries is that the framework sequences to be used are predetermined. This will help the development and application of specific antibodies. The semi-synthetic library is carefully designed by assuming the number of scaffolds, the positions to be diversified, the types of amino acids to be included in the design, and the ratio of amino acids to be diversified in each position. These assumptions are not always correct. In order to avoid making assumptions about the structure of the CDR, a combination of synthetic scaffolds and natural CDRs was used.
Creative Biolabs has many years of experience in the development of semi-synthetic antibody libraries. We use phage as vectors to produce high-affinity custom antibody libraries, with industry-leading titer assurance and quality assurance. Our services are listed as follows, but are not limited to:
The factors that affect the effective size of the antibody library are as follows:
Creative Biolabs can meet the needs of customers for providing semi-synthetic library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
