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Phage Display Synthetic Library Construction

Synthetic Libraries Construction Services with Phage Display

Synthetic antibody libraries help design, construct and successfully generate high-functional antibodies suitable for various applications. Compared with natural antibodies, its optimized framework sequence (strong scaffold) and controlled CDR diversity make it have unique advantages. Phage display is a technique widely used to study protein-protein, protein-peptide and protein-DNA interactions. With the development of antibody drugs and vaccines, phage display libraries have had a revolutionary impact on antibody engineering. It makes it possible to screen and identify billions of target binding proteins in a short time. Creative Biolabs uses the M13 single-chain filamentous phage display system to co-express recombinant proteins or antibodies with phage pIII protein. With the help of helper phage, protein or antibody will participate in phage assembly and display on the phage surface, forming an antibody phage display library.

Our Services

Creative Biolabs' phage display technology has been successfully applied to the construction of synthetic antibody libraries. We can also provide flexible and efficient customized services according to customers' special needs. Our services are listed as follows, but are not limited to:

  • Library design
  • Construction of synthetic antibody phage-display libraries
  • ELISA screening
  • DNA extraction and antibody sequencing
  • Library QC

Services Workflow

  • Obtain sequence: obtain antibody sequence from RT-PCR and sequencing, database
  • Vector construction: clone in the phage display vector
  • Transform E.coli
  • Antibody library construction and production
  • Titering: collect antibody phages and determine the titer of the phage
  • Enrichment of screening
  • Phage ELISA (Optional)
  • Sequencing
Synthetic Libraries Construction Services Workflow

Delivery Result

  • Synthesized variable fragment
  • Variable fragment phagemid library and COA
  • Result analysis reports
  • Format: scFv, Fab, VHH

Our Advantages

  • Precise targeting: Control the codon preference and amino acid composition, as well as the ratio of each mutation site in the CDR.
  • High diversity: The exquisite design creates a high diversity of synthetic antibody libraries.
  • High-quality library: The updated phage display system provides a guarantee for larger storage capacity and high-functional antibodies.
  • Cost-effectiveness: competitive prices and affordable services.

Creative Biolabs can meet the needs of customers for providing synthetic library construction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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