After obtaining a high-titer lysate of the cloned phage population, you can further characterize the phage by analyzing its genome. Genome is defined as the complete set of the genetic material of an organism, including all its genes and regulatory sequences. To characterize the phage DNA, it is necessary to extract it from the phage lysate. After separation, you can use restriction enzyme digestion and DNA sequencing experiments to study the phage DNA. The lysate contains cloned phage population and the remnants of lysed bacterial cells, including bacterial nucleic acids (DNA and RNA), proteins, and lipids. Since the presence of bacterial DNA and RNA will interfere with future experiments aimed at studying phage genomes, enzymes called nucleases are used to destroy bacterial nucleic acids in DNA extraction. the phage genomic DNA is surrounded by the protein shell of the phage capsid, so it is protected from nucleases. After a short incubation period, the nuclease is inactivated by adding a denaturant. Once the phage genomic DNA is released, you can purify it from the remaining lysate.
We provide a fast spin-column method for the purification of total DNA from a broad spectrum of phage propagating from bacteria growing in liquid culture. There is no need to use phenol, chloroform, or cesium chloride banding procedures in DNA isolation. The procedure based on the spin column is very fast and can be completed in a short time. The purified total phage DNA has the highest integrity and can be used in a variety of downstream applications, including PCR, qPCR, restriction fragment length polymorphism (RFLP), sequencing, cloning, Southern Blot, etc.
Creative Biolabs can meet the needs of customers for providing phage DNA extraction services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
