The phage DNA vaccine consists of a eukaryotic expression cassette that encodes a specific antigen controlled by a target-specific promoter contained in the phage. The expression cassette must have the necessary regulatory sequences to allow correct gene expression and protein folding of the antigen. In this strategy, phage particles act as passive vectors, allowing the DNA-encoded antigen to be transferred to eukaryotic cells, where the DNA is expressed. Phage DNA vaccines are considered to be more advantageous than standard DNA vaccines because the former is more stable in storage, transportation, and administration (mainly via oral routes) because the capsid of the phage particles protects the DNA from degradation. Although ʎ phage is the most common phage vector used for DNA vaccination, filamentous phages have also been tested. An important feature to consider for filamentous phage-DNA vaccines is that the phagemid vector can effectively contain multiple gene copies, which may allow the use of a single delivery vector to immunize various epitopes or antigens. Compared with the naked DNA strategy, the phage DNA vaccine can induce more effective immune protection.
Fig 1. Antigen identification using phage display technology
Creative Biolabs can provide high-quality phage DNA vaccines development services. We provide different quantitative analyses and qualitative analyses to evaluate phage DNA vaccines according to strict requirements. Our services are listed below, but not limited to:
Creative Biolabs can meet the needs of customers by providing vaccine development services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
