Bacteriophages are very abundant in soil, and the direct counting method found that there are 107-109 phages per gram of soil. Viruses are also very abundant in fresh water and seawater, which may be close to 107 per milliliter. The global number is estimated to be 1031. However, certain phages are sometimes difficult to isolate directly, so enrichment procedures are usually required. Unfortunately, this technique will limit the diversity of isolated phages, because any enrichment scheme will lead to bias, that is, phages that can reproduce most efficiently under experimental conditions dominate. At Creative Biolabs, we can build different bacterial models to enrich the phages from the soil. For example, we used Bacillus as a model bacteria to enrich phages from the soil. Our team has advanced technology and rich experience and can provide customers with the most suitable solutions to meet your project needs.
The phages enrichment from soil services we provided can use filtered extracts that do not contain contaminated bacteria as the basis for the growth of indicator bacteria to provide the success rate of isolation. By first removing the soil bacteria, the target phage is not hindered by competition with the natural soil bacteria present in the original soil sample. This enrichment method has produced dozens of unique phages from several different soil types, and even different types of phages from the same enriched soil sample isolate.
Collect 200-400 grams of soil from the desired location.
Our phage isolation methods include direct isolation of phage and enriched isolation of phage.
Our phage purification methods include PEG precipitation, chloroform extraction, anion-exchange chromatography and size-exclusion chromatography.
Creative Biolabs can meet the needs of customers by providing phage enrichment from the soil on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
