Phage Genome Sequencing

Bacteriophages are viruses or semi-autonomous genetic entities that rely on prokaryotic cell metabolism for reproduction. In many fields such as food preservation, disease control, and agricultural production, lysed bacteriophages are used as biological control agents, but the most appropriate and standardized methods are required to ensure application safety. At present, a large number of phage genomes are sequenced, and researchers apply genome comparison and other methods in phage classification or typing. different phages of the same host bacteria have huge differences in morphology, biological characteristics, and genomes. phages isolated from the same species with similar phenotypes showed polymorphism in their genomes, and even phages isolated from the same host bacteria are much more different than phages isolated from different species in genetic. Sometimes the gene sequences of bacteriophages with different morphologies or biological phenotypes are similar. Creative Biolabs provides phage gene sequencing services to meet customers' specific needs and achieve customers' research goals.

Our Services

Creative Biolabs provides phage sequencing services include whole-genome sequencing, high-throughput sequencing in order to obtain the annotated genome of your phage. Phage DNA is purified using a specific DNA isolation protocol, and the remaining bacterial DNA contamination is removed by bioinformatics after sequencing. Our services are listed as follows, but are not limited to:

• Whole-genome sequencing
  The platform of Creative Biolabs has great potential in phage genome sequencing. The comprehensive phage sequence will facilitate the interpretation of phage metagenomics data by providing reference genomes, thereby better understanding phage diversity, ecology, adaptability, and evolution.
• High-throughput sequencing
  High-throughput sequencing (HTS) technologies are rapidly improving in terms of quality, speed, and cost. High-throughput sequencing is a newly invented alternative to microarray technology. Creative Biolabs can provide HTS service based on your phage sample.
• Phage characterization
  Creative Biolabs provides phage characterization services through whole genome sequencing uses high-throughput sequencing to obtain the annotated genomes of your phages.
• Bioinformatic analysis
  Creative Biolabs provides bioinformatic analysis service on our phage technology platform, and our services include raw data quality control, statistics of sequencing depth and coverage, de novo assembly, reference genome mapping and population genetics analysis, and so on.

What You Receive

• A report with the method followed.
• Original high-throughput sequencing files.
• The results of bioinformatics analysis include:
• Demultiplexing of raw data
• Preprocessing and quality control
• Removal of bacterial reads
• De novo assembly
• Removal of duplicate reads
• Automatic gene annotation
• Average nucleosides between the genomes sequenced and publicly available reference genomes

Service Flow

Creative Biolabs can meet the needs of customers for providing phage isolation services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).