Tetracysteine tags constitute an invaluable tool for gaining a deeper understanding of the mechanistic processes that control virus-host interactions. Phages use cellular machinery to enter host cells and initiate their replication cycle. The development of methods to visualize phage entry into living cells has provided new insights into the cellular processes involved in phage entry and the intracellular location of phage payload deposition. Currently, the use of fluorescently labeled phages and high-resolution microscopy is the method of choice for studying phage entry into living cells. Labeling phage proteins containing a small tetracysteine (tc) tag with bis-arsenic fluorescent compounds (e.g., FlAsH, ReAsH, Lumio-x) offers several advantages over traditional xFP fusion constructs. Creative Biolabs has a long and successful history of providing phage engineering solutions to accelerate biopharmaceutical development and overcome a variety of challenges. Creative Biolabs' portfolio of fluorescent labeling and phage engineering services offers a range of standard and custom packages. We pride ourselves on being able to meet customer needs. Our standard set-up mixes of facilities, equipment, protocols, support systems, and people is all but guaranteed to help!
Creative Biolabs works with companies and academic groups around the world, including most of the top 20 biopharmaceutical companies, to support better phage development options for researchers. Creative Biolabs has a group of scientists with an outstanding understanding of the phage genome. Our scientists successfully designed several different phages, each with a different tag, and applied a strategy to label the phages. Our services are listed as follows, but are not limited to:
The development of synthetic phage products targeting specific pathogens begins with the isolation of robust natural phages from environmental and clinical samples. Creative Biolabs' large library of multidrug-resistant pathogens and microbiome targets helps identify optimal phage candidates for downstream engineering.
We have developed several label-based phage detection methods, employing different labeling strategies for different methods, including one that visualizes phages by labeling their nucleic acid genomes, and the other that detects bacterial targets using phage labeling primarily by phage component labeling.
Creative Biolabs can meet the needs of customers by providing phage-integrated tetracysteine tagging design and production on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
