The multiplicity of infection (MOI) is the number of phage particles that are added to each host cell to initiate infection and thereby produce. Small-scale or a few milliliter methods developed using phage λ and M13 generally tend to use high MOIs of 5-10 or higher. MOI is important for amplification because it defines how large a phage culture needs to be provided for amplification. It is desirable nor sensible to have to cultivate the phage fermenter in the final preparation to inoculate a slightly larger fermenter. Similarly, when the MOI is less than 10, we have reached the point where every cell has enough phages to infect them. Under this MOI, we can only expect a single burst of phage particles to lyse the phage and therefore only a small increase in the number of phages added. The host of phage infection is generally bacteria, fungi, algae, actinomycetes, or spirochetes. When MOI is 1, only 63% of cells will be infected by one or more phages, because MOI has a poisson distribution to infected cells.
We uphold the customer-centric tenet and are committed to providing phage custom services and saving customers a lot of time at competitive price. At Creative Biolabs, we provide the determination of MOI of phage and host. The services are listed as follows:
The range of MOI to be used in the design, for example, 6 conditions ranging from MOI 1, 2, 5, 10, 15 and 30.
After infecting the cells, allow an appropriate amount of time before evaluating the fluorescence. Record the fluorescence at various MOIs to determine the percentage of your transduced cells.
If your cells are inherently difficult to transduce, you can use transduction enhancers such as polybrene.
Creative Biolabs can meet the needs of customers for providing phage MOI determination services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
