The most abundant life forms, phages, have had a major impact on the biosphere, bacterial evolution, bacterial genomes, and horizontal gene transfer. Importantly, phages have been and continue to serve as valuable model systems. These studies have led to renewed interest and activity in the study of phages and their genomes. To determine the details of phage involvement in these important processes and activities, the assignment of specific functions to phage gene products is critical. Initial function and gene assignments can be made by general mutagenesis of the phage genome and these specific gene products. A very useful mutagenesis protocol that has revived interest is the use of hydroxylamine. A mutagenesis protocol has been used to obtain genetic mutations associated with the lysogenic cycle of Salmonella enterica serotype Anatum var. 15+ phage epsilon 34 and isolates conditional lethal mutants of phage epsilon 34. Researchers propose a plate complementation method to rapidly determine the number of genes present in mutant populations isolated from hydroxylamine mutagenesis.
The mutants of phages offers a particular advantage in treating bacterial infections that other antimicrobial therapies do not offer. We provide phage mutant construction services. They are listed as follows, but are not limited to:
The use of hydroxylamine (HA) has a long and rich tradition in gene identification, gene product analysis, and gene regulation. The reason for this tradition is the nature of mutation, which results in irreversible single nucleotide substitutions from cytosine → thymine. There are two steps to the action of hydroxylamine. The first step depends on the metal passivation process. The second step mutates the phage, but rather slowly. Inclusion of a chelating agent eliminates the first step and makes mutagenesis a first-order reaction and an extremely effective tool.
Creative Biolabs can meet the needs of customers by providing phage mutants construction services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
