P22 is a bacteriophage of Salmonella typhimurium with a circular arrangement of terminally redundant chromosomes. P22 can be integrated into and excised out of the host chromosome by site-specific recombination. One of the key steps in the life cycle of bacteriophage P22 is the circularization of infected linear chromosomes, which is caused by phage recombination at the terminal repeat ends of the bacteriophage DNA system facilitated. This circularization mechanism differs from that seen with phage l, where circularization proceeds by annealing of overlapping single-stranded cos (sticky end) sites. This distinction makes recombination an essential feature of P22 biology, whereas phage I is not. However, similar to l, phage P22 produces tandem DNA for packaging. Like phage l, phage P22 produces chromosomal multimers in two ways: phage-promoted recombination events between two monomeric loops and a rolling circle pattern of DNA replication.
P22 shares many similarities with bacteriophage λ in its genetic structure and regulation. It is a temperate double-stranded DNA phage as well as lambophage because it has a similar gene expression region and early operon control to phage λ. Our researchers have developed a recombination phage platform technology that can be easily and precisely engineered for specific phage applications. We provide construction services of phage P22 recombination system construction services. They are listed as follows, but are not limited to:
The recombinant system of P22 consists of four proteins: Erf, Abc1, Abc2 and Arf.
P22 has been used for transduction in S. enterica sv. Typhimurium. An important factor in the development of S. enterica genetics is the ease of use of P22 for transduction hybridization. In particular, P22 is storage-stable, high-titer stocks are readily available, and high-frequency transduction (HT) and integration-deficient mutants have been isolated.
Creative Biolabs can meet the needs of customers by providing construction services of phage P22 recombination system on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
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