In phage research and treatment, most applications require highly purified phage suspensions. The standard technique is ultracentrifugation using a cesium chloride gradient. This technique is cumbersome, complicated, and expensive. In addition, it is not suitable for the purification of large quantities of phage suspensions. Anion exchange chromatography is a popular technique for the purification of peptides, proteins, oligonucleotides, nucleic acids, and charged molecules. The popularity of this chromatography is related to its high resolution, high capacity, simplicity, and controllability. Especially, the M13 phage has a net negative charge at neutral pH, an anion exchange adsorbent is selected and a purification process using a fast protein liquid chromatography (FPLC) system will be established. With the help of anion exchange chromatography and knowledge about the structure of phage, a fast and simple method was developed by our scientists for recovering phages from clarified bacterial cultures.
A suitable purification method depends on your phage project. If you have questions about which purification method is suitable for your project, please feel free to contact our experts and they can provide guidance. Our services are listed as following, but are not limited to:
Creative Biolabs can meet the needs of customers for providing phage purification services with anion-exchange chromatography on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
