Cesium chloride (CsCl) is a dense salt that can form a linear gradient when ultracentrifugal force is applied to a uniform suspension (such as cell lysate). There is no need to form a gradient in advance. The CsCl gradient is usually steep, and the maximum density within the gradient usually exceeds everything except the densest substance to be precipitated, which means that higher density molecules will collect as particles at the bottom of the tube. Purification of phage by CsCl density gradients usually requires expensive ultracentrifuge and is a basic technique in virology. The CsCl density gradient method generally requires ultracentrifugation at more than 100,000×g, which cannot be achieved by ordinary centrifuges. Since phage purification via CsCl density gradient ultracentrifugation requires an expensive ultracentrifuge, rotor, and special tubes, not all suppliers can use this powerful method. At Creative Biolabs, we have the most advanced equipment and technology to provide customers with one-stop service for phage research.
However, some bacteriophages degrade the host's DNA, but not consistently to a safe level. Proteases should be removed because they will negatively affect the shelf life of the phage. Creative Biolabs has accumulated rich experience in providing phage purification services using CsCl gradient centrifugation. Our services are listed as follows, but are not limited to:
Creative Biolabs can meet the needs of customers for providing phage purification services with CsCl gradient centrifugation on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
