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Phage Purification with PEG Precipitation

Phage Purification with PEG Precipitation

Polyethylene glycol (PEG) has strong hydrophilicity, is easily soluble in water and many organic solvents, has good thermal stability, and has a wide molecular weight range. In the polyethylene glycol precipitation method, PEG is formulated into a 50% solution, or solid PEG is directly added to the virus suspension to reach the desired concentration, usually stirred at 4°C overnight, and then centrifuged to precipitate the virus. The precipitation effect of PEG is mainly related to its own concentration and molecular weight, but it is also affected by factors such as ionic strength, solution pH, and temperature. PEG with a molecular weight of 2000 to 6000 is mainly used for virus precipitation. Creative Biolabs, with years of research experience in phage and advanced equipment, can provide customers with phage precipitation solutions, a simple and efficient method to concentrate phage particles.

Our Services

The purification method you need depends on your phage development project. Creative Biolabs has a large number of advanced equipment, professional technicians. And our expert team can provide you with the required phage purification services. Our services are listed as following, but are not limited to:

  • Phage propagation
  • Lysate preparation
  • Phage concentration
  • Phage purification with PEG precipitation
  • MS data provided
  • Providing ultracentrifuge
  • Process development
  • Traceability analysis
  • Monitor phage quality
  • Evaluate purification efficiency
  • PEG precipitation optimization
  • Phage lysates

Purification Materials Required

  • PEG Solution
  • Phage re-suspension solution
  • Instruments and reagents for purification

Services Workflow

  • Preparation of phage suspension
  • Phage culture proliferation
  • Prepare lysate
  • PEG precipitation
  • Phage detection

Highlights

  • Fast: the whole journey only takes 30 minutes
  • High efficiency: It can concentrate phage particles 10-100 times
  • Safe: non-toxic to all target cells, including embryonic stem cells
  • Easy to use: no need to use special equipment, avoid the use of expensive ultra-high-speed centrifuges, just ordinary centrifuges, low-speed centrifugation can be no residue
  • No debris:no residues of cell debris, avoiding cell toxicity and immunogenicity

We Do Better

Phage Purification with PEG Precipitation
  • Excellent service
  • Dedication to quality
  • Extensive experience
  • Open communication
  • Competitive price
  • Cutting edge science
  • Global Delivery

Creative Biolabs can meet the needs of customers for providing phage purification services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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