Size Exclusion Chromatography (SEC) is currently the most powerful chromatography technique that can be used to obtain reliable information about the size of biomolecules under natural conditions. Phage display has proven to be a powerful and convenient method for rapid sampling of target ligands for billions of protein structures. The most common system uses protein and peptide display as a fusion to coat protein III or VIII of the M13/fd phage. Effective screening of phage display libraries requires high purity of input phage particles. Polyethylene glycol (PEG) double precipitation (PEG-8000) with an average molecular weight of 8000 Da is the standard technique for purifying filamentous bacteriophages. Some contaminants may remain in the PEG-purified phage preparation. Size exclusion chromatography has been used to prepare pure eukaryotic viruses and bacteriophages lambda. SEC is the preferred method for the effective purification of filamentous bacteriophages under mild conditions.
SEC is an efficient method used to purify your phage. The purification method you need depends on your project. Our services are listed as follows, but are not limited to:
The panning efficiency of SEC-purified phage is analyzed in three different formats:
Creative Biolabs can meet the needs of customers for providing phage purification services with size-exclusion chromatography on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
