Bacteriophage recombineering of electroporated DNA (BRED) for simple and efficient construction of targeted phage mutants. BRED can be used to construct untagged deletions of essential and non-essential genes, in-frame deletions, point and nonsense mutations, addition of gene tags, and precise insertion of foreign genes. This technique is applicable to mycobacteriophages and should be generally applicable to phages of other hosts for which recombinant systems are available. It involves the co-electroporation of recombination substrates (i.e., phage DNA and double-stranded DNA (dsDNA)) into electrocompetent bacterial cells carrying plasmids encoding proteins that promote high levels of homologous recombination, such as RecE/RecT-like proteins. By using this method, modified phages can be obtained at high frequency (10% to 15%), allowing screening of putative mutants by small amounts of PCR without further selection. This technique requires a highly competent bacterial host.
Creative Biolabs successfully obtained phage genetic modification using bacteriophage recombineering of electroporated DNA. With the accumulation of technology and resources, the reconstruction of the microbial genome is to realize the research and utilization of genes by interrupting, inserting, or deleting genes in the microbial genome.
We are months ahead of our competitors. We start projects within a day of client approval and complete pilot studies within 15-20 days. Subsequent large-scale phage recombineering takes 5-10 days, and product isolation is usually completed after 2-3 days. Our typical project timeline from start to finish is five weeks.
Creative Biolabs can meet the needs of customers by providing bacteriophage recombineering of electroporated DNA services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.