By spreading a thin layer of bacteria, culture medium, and agar on the plate, and moving a small aliquot of the sample to be tested to the top, the enriched isolated culture is screened for the presence of phages. As the bacterial lawn grows, the phages present in the sample will complete the cycle of infection and lysis, resulting in a large area of clearance or "spots" composed of multiple overlapping plaques. Therefore, the Spot test is a method to screen multiple samples of phage on the same plate. This test will determine whether the putative plaque will multiply phages. It is a good time to test for the presence of phage in the sample using this method after enriched isolation samples collection. There is no need to use serial dilutions for plaque detection, only a single plate can be used for a spot inspection.
Spot test is a quick way to check whether a sample of bacterial cells can be infected with bacteria. It will be a small drop of bacterial cells or the bacterial cells are spotted on the plate where the bacteria are infected. At Creative Biolabs, our scientists can provide high-quality services to meet your needs. Our services are listed as follows, but are not limited to:
Creative Biolabs can meet the needs of customers for providing phage spot test services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
