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Phages are mandatory intracellular parasites that enter suitable host cells and usurp their functions to promote self-replication. Therefore, the phage life cycle includes a series of complex interactions between the phage and the host cell's protein, structure, and machinery. The characteristics of these interactions are not only qualitative but also quantitative, which is essential for a true understanding of phage replication. We use isotopic labeling and fluorescent labeling for phage research. Isotope labeling is a technique used to track isotopes through reactions, metabolic pathways, or cell pathways. Reactants are "labeled" by replacing specific atoms with their isotopes. Fluorescent labeling technology is a technology that connects the covalent bond of a fluorescent group to proteins, nucleic acids, and other molecular substances that can recognize molecules. The technology uses the specific group of the fluorescent signal substance to react with the specific group of the recognition molecular substance to complete the labeling process, and use the fluorescent properties of the label to provide the signal of the detected object.
At Creative Biolabs, our team provides isotope labeling and fluorescent labeling of phage to study the replication of phage in the host and the interaction between phage and host. Our services are listed as follows, but are not limited to:
Our expert team uses 15N, 32P, and 35S to label the phage and let the phage infect the bacteria, and the radioactive elements can be found in the structural components of the progeny phage produced.
Since phages and viruses are basically composed of protein-encapsulated DNA or RNA, they are also suitable for fluorescent labeling, allowing researchers to count and track them. Furthermore, fluorescently labeled phages can be used to track and identify their host bacteria.
Creative Biolabs can meet the needs of customers for providing phage tagging services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.