Biosensors have been developed as new diagnostic methods to minimize the limitations of common pathogen detection methods. High specificity is one of the main characteristics of biosensors in sensitivity-assisted diagnosis, and there is no need to spend time in the pre-enrichment stage. In recent years, phage-based biosensors have been developed, which have excellent robustness and selectivity, and are easy to integrate into transduction devices. In the phage-based biosensor, the phage is attached to the surface of the sensor, so the pathogen in the sample can be detected. The main advantages of this method are sensitivity, accuracy and reliability. Bacteriophages are specific to host bacteria and have different characteristics, including easy amplification, low production cost, resistance to temperature and pH degradation, and organic solvents. In addition, the cloning of engineered phage from the phage display library enables the development of new phage with on-demand binding units on its surface. This makes bacteriophages a very promising candidate for biosensor applications.
Fig 1. Scheme representation of electrochemical detection of E. coli using engineered phage. (Wang D, et al. 2017)
Traditional biochemical and microbiological tests are expensive, complicated, time-consuming, and not always reliable. At Creative Biolabs, our science provides simple, cheap, fast, sensitive, specific and reliable biosensor design and development. Our biosensors types are listed as follows, but are not limited to:
At Creative Biolabs, the current methods for engineering of phage can be divided into three categories: (i) direct cloning; (ii) homologous recombination with or without CRISPR-Cas counter selection; (iii) whole genome activation.
According to the genetic incorporation of functional scaffolds or the display of engineered peptides, proteins and antibodies, phages as biosensing probes can be divided into phage receptor-binding protein bioprobes, recognition elements and reporter phages
Phage itself can participate in sensing events, not only as a support for probe recognition, but also to provide phage structural elements.
In order to effectively attach to the surface of the bacterial host, the phage targets the bacterial cell surface receptor through a receptor binding protein located in the tail fiber of the phage.
Reporter phage is a genetically modified phage used as a gene carrier to introduce the gene of interest into a specific bacterial genome.
Creative Biolabs can meet the needs of customers by providing biosensors development solutions on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
