Phages are a class of viruses that are obligately parasitizing microorganisms such as bacteria and actinomycetes. Their sizes are extremely small, and their amounts cannot be measured by conventional microbial counting methods. Phages are ubiquitous organisms, and they can usually be found in places full of bacterial communities, such as soil and the internal organs of animals.
The phage titer is the number of infectious phage particles contained in a 1 mL sample. The determination of phage titer generally adopts the double-layer agar plate method. Since a phage generally produces a plaque on an agar plate containing a specific host bacterium, the phage titer can be calculated based on the number of plaques appearing in a certain volume of phage culture solution. The plaques formed by this method are more consistent in shape and size, so the count is more accurate, and it is widely applied in phage researches. Learning about the characteristics of phages, rapid inspection, separation, and titer determination play an important role in preventing phage contamination in production and scientific research.
With many years experience in phage research, we are the best choice to provide custom phage testing services on time and on budget. Creative Biolabs provides phage titer testing services and our services are listed as follows, but are not limited to:
The determination of phage titer generally adopts the double-layer agar method. The method is to use a layer of the solid medium as the bottom medium, then mix the host bacteria, phage dilution, and semi-solid medium (in a molten state), and pour it into the upper plate to cool. After solidification, place it in an incubator, and count the number of appearing plaques.
Creative Biolabs can meet the needs of customers for providing phage titer test services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
