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Phage Typing

Phage TypingThe specificity of bacteriophages for bacterial cells makes it useful for the typing of bacterial strains and the detection of pathogenic bacteria. Phage typing is also known as the use of sensitivity patterns to specific phages to accurately identify microbial strains. The sensitivity of the detection will be increased,if the specific antibody detects the phage that binds to the bacteria. For the detection of unknown bacterial species, different phages are set on the lawn. If plaques (transparent areas) appear, it means that the phages have grown and lysed the bacteria, making it easy to identify specific bacterial species. There are certainly other methods that can be used to detect pathogens, such as the use of bacteriophages, which can specifically deliver reporter genes or use the green fluorescent protein that is expressed after bacterial infection. Similarly, a phage with a fluorescent dye covalently attached to its coating can be used to detect specific adsorption. It is also possible to detect some released components, such as adenylate kinase after specific lysis of bacteria, and antibodies and peptides displayed by phage, which will specifically bind toxins and bacterial pathogens.

Bacterial type assignation via lysis by a set of standardized typing phage.Fig 1. Bacterial type assignation via lysis by a set of standardized typing phage. (Kirchhelle C, 2020)

Our Phage Typing Services

Creative Biolabs provides some of the most comprehensive services in the world to detect the presence of bacteriophages and prophages in various bacterial hosts. As a phage professional laboratory and world expert, with access to a complete phage array, Creative Biolabs is particularly suitable for performing phage typing which is used phage to identify bacterial strains and track the source of bacterial infection or pollution outbreaks. Our services are listed as following, but are not limited to:

  • Phage adenylate kinase assay
  • Mycobacterium tuberculosis detection
  • E.coli, Pseudomonas detection
  • Salmonella, Listeria detection
  • Campylobacter detection

Dual Phage Technology

We use dual phage technology to detect the binding of antibodies to specific antigens. Phage amplification test can also be used to detect pathogens. This technology is most widely used to detect Mycobacterium tuberculosis, E.coli, Pseudomonas, Salmonella, Listeria and Campylobacter.

Applications of Phage Typing

  • Phage detection has long been the gold standard method for monitoring Salmonella typhimurium or Salmonella enteritidis.
  • Phage typing is used to determine the relatedness of bacterial species by microbiologists.
  • Phage typing is the most widely recognized typing method for Staphylococcus aureus, and it is still widely used to subdivide the serotype of Pseudomonas aeruginosa.
  • Phage typing is still an application based on laboratory genotyping technology, especially in the pollution control of biological processes.

Our Advantages of Phage Typing

  • In terms of bacterial phage typing, we can provide a set of different phages, a large number of technical expertise, and the need to carefully control the test environment.
  • Creative Biolabs is one of the few laboratories in the world with all these attributes, methods, facilities, and well-trained personnel.
  • Creative Biolabs can perform phage typing for any type of industrial application or biological process.

Creative Biolabs can meet the needs of customers for providing phage typing services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.

Reference

  • Kirchhelle C. The forgotten typers: The rise and fall of Weimar bacteriophage-typing (1921–1935)[J]. Notes and Records, 2020, 74(4): 539-565.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Biophage Technology

Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.

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