Gene delivery has been described as the Achilles heel of gene therapy. Vectors for gene therapy must be able to efficiently deliver foreign genes to specific cells with minimal toxicity. Safety is a major concern for animal viral vectors. The preparation of animal viral vectors must be free of replication-competent viruses, making large-scale economic production a major challenge. Phage vectors possess many desirable properties of animal viral and non-viral systems. Like non-viral vectors, phage particles are simple genetic packages that lack intrinsic tropism for animal cells. However, phages can be produced at high titers in bacterial culture, which may be more superficial and more economical than non-viral or viral systems. Phage particles are also very stable under various harsh conditions including extreme pH, DNase, and proteolytic enzymes. Filamentous phages allow a variety of proteins, antibodies, and peptides to be displayed on the phage coat, allowing the phage to genetically target cell surface receptors. Furthermore, the ability to perform directed evolution of a large number of phage variants enables genetic selection for improved phages due to their ability to target and transduce cells in specific tissues.
Fig 1. Construction of phagemid and phage vectors. (William J, et al., 2013) Phage vectors can be designed for receptor-mediated gene transfer into mammalian cells. The attractive properties offered by this system paved the way for various attempts to develop phages as vectors for gene therapy applications. Creative Biolabs provides phage vector development for gene delivery relying on our powerful DNA sequencing, synthesis and engineering technology platform.
One of our most widely used expression vectors is E. coli because of its ease of growth, rapid propagation, and ease of transformation with foreign DNA.
Phage vector-based vaccines constitute a promising segment of the biopharmaceutical pipeline, addressing many unmet indications. To harness the full potential of phage vector-based vaccine development, our team is seeking innovative technologies to support more efficient, enhanced, and flexible processes.
At Creative Biolabs, advanced chemometric methods, such as experimental design and multivariate data analysis, provide extensive data visibility, ultimately increasing process reliability and robustness while saving costs.
The phage vector consists of a complete phage genome, and we usually use the M13 phage and the λ phage. The λ phage vector has the advantage of high transformation efficiency due to good commercial packaging extracts. The types of λ phage vector are listed as follows:
Creative Biolabs can meet the needs of customers by providing phage vectors development for gene delivery on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
