Prophage plays an important role in prokaryotic evolution, usually by transferring new genes (such as virulence or antibiotic resistance factors), inactivating existing genes, or modifying gene expression to change the function of the cells they infect. Recently, phage therapy has regained interest as a promising alternative method of controlling bacterial infections. The cataloging of prophages in the genomes of a large number of species is an important initial step to understand their evolution and potential therapeutic utility. However, the currently widely used tools for identifying prophage in bacterial genome sequences are mainly network-based, and the response time may be very long, and they cannot keep up with the current routinely sequenced thousands of genomes. Creative Biolabs has the skills and capabilities to detect toxic phages and prophage, derived from the professional knowledge of the company's founders, and they have also participated in many aspects of the development of the toxic phage and prophage induction in a variety of bacterial strains.
Prophage is the "pre" form of Phage: the initial stage before the virus inserts into the host's DNA in genomic form and then attaining its real form inside the host. Therefore, a prophage in the genome of a phage, which can exist as an extrachromosomal plasmid or be inserted and integrated into the circular bacterial DNA chromosome. This latent form of phage stores its viral genes in the bacteria without destroying the bacterial cells. This potential form makes it more difficult to detect.
| Technology Platform | Content |
|---|---|
| Sanger sequencing | Sanger sequencing efficiently and quickly identifies virus mutations. It is a reference method for viral genome sequencing and can determine the source of antiviral drug resistance. |
| Next-Gen small genome sequencing | Next-generation sequencing of viral genomes is a reliable technology that provides a faster and more sensitive method than Sanger sequencing. |
| DNA/RNA sequencing | Before the sequencing reaction, the phage DNA or RNA is separated from the phage particles, and then PCR or RT-PCR is performed. |
Creative Biolabs can meet the needs of customers for providing prophage test services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
