Many studies have confirmed that ultraviolet radiation can produce a variety of active oxygen. They are very active, have strong oxidizing properties, and can cause damage to biological macromolecules such as DNA and proteins. For example, ultraviolet light can cause many forms of DNA damage. Irradiating the lysogen with an appropriate dose of ultraviolet light can effectively induce the prophage to enter the lysis pathway. At Creative Biolabs, this study established two new methods, namely the colony counting method and the plate induction method, to determine the ultraviolet induction rate of the prophage. The lysogen cultured in the dark after induction by ultraviolet irradiation was directly spread on the plate, and the induction rate of prophage is calculated by CFU (colony forming unit). A plate containing a mixture of lysogen and indicator bacteria is induced by ultraviolet irradiation and calculation of the induction rate of prophage is based on PFU (plaque-forming unit). These two methods can not only accurately determine the frequency of phage induction, but are also simple to operate, labor-saving and instrument-saving, and easy to reuse.
Our phage analysis team provides customers with phage custom services about prophage induction determination. With the help of our powerful analysis capabilities and equipment in phages, we can provide global customers with competitive services and significantly reduce customer development costs. We have established the listed methods to determine the induction of prophage under ultraviolet (UV). Our methods are listed as follows, but are not limited to:
The soft agar plate method not only requires a lot of work, but also has low potency and yield.
Although the colony counting method is more complicated to operate, but it can obtain information about live bacteria, so it is widely used in the detection of phage contamination.
The plate induction method can quickly and accurately determine the ultraviolet induction rate of the prophage.
Creative Biolabs can meet the needs of customers for providing prophage UV induction determination services on time and on budget. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each professional for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
