Directed evolution (DE) is a method used in protein engineering, which simulates the process of natural selection to guide proteins or nucleic acids to user-defined targets. It includes repeated rounds of mutagenesis of genes, selection, and amplification. It can be performed in vivo or in vitro. Directed evolution is used in protein engineering as an alternative to the rational design of modified proteins, as well as experimental evolution studies of basic evolution principles in a controlled laboratory environment. Targeted transformation protein refers to the use of cassette mutation and error-prone PCR methods to transform mutant proteins or specific cod sequence domains. The existing protein or domain mutation library will be displayed on the surface of the phage. Then we can obtain the desired phage clones with directed changes through affinity screening. The primary structure can be derived from DNA sequences and can be used to screen more cytokine receptor capabilities, new enzyme inhibitors, DNA transcription factors and new site combinations, new cytokine antagonists, new enzymes and enhanced biologically active proteins. Phage display technology as a selection-based system, it is an attractive method for the development of new biopharmaceuticals.
Fig 1. The process of directed evolution in the laboratory mimics that of biological evolution. (Packer M S, 2015)
Creative Biolabs can provide customers with one-stop protein directed evolution services on phage display paltform according to their different needs. Our services are listed as follows, but are not limited to:
Use synthetic biology methods to construct protein or regulatory sequence site-directed mutagenesis libraries to form different mutants.
Random mutation service platform developed by Creative Biolabs. Using our own reagents and systems can effectively introduce nucleic acid mutations.
Researchers can reset different regulatory elements or genetic pathways according to their own designs to create new transcription units, biochemical pathways, genetic circuits or engineered bacteria.
It is an ideal tool for biologists to determine the minimum stable domain of crystallization and determine the boundary amino acid residues that have important functions for proteins.
Creative Biolabs can meet the needs of customers by providing protein directed evolution services on time and on budget. We have in-depth knowledge and experience of the tools and processes involved in the phage projects. Our skilled and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for your project. If necessary, please feel free to contact us.
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
